PCR Equipment and Supplies

A wide variety of products designed for use in various PCR procedures. Includes thermal cyclers, oligonucleotides, dNTPs, enzymes, assays, arrays, master mixes, reagents, kits, etc.

91 – 105 of 6,055 results

Thermo Scientific™ Fluorescein-12-dUTP Solution (1 mM)

Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.

Pricing & Availability

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific RevertAid H Minus Reverse Transcriptase is a recombinant M-MuLV RT. The enzyme possesses an RNA-dependent and DNA-dependent polymerase activity, but lacks RNase H activity due to a point mutation in the RNase H domain.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	• RevertAid H Minus Reverse Transcriptase (200 U/μL) • 5X Reaction Buffer Store at –20°C.
Reverse Transcriptase	RevertAid H Minus
Final Product Type	First-Strand cDNA
Concentration	200 U/μL
Format	Stand-alone Enzyme
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C to 45°C
Technique	Reverse Transcription
Starting Material	RNA

Thermo Scientific™ M13/pUC Sequencing Primer (-40), 17-mer

Thermo Scientific M13/pUC Sequencing Primer (-40), 17-mer is a synthetic single-stranded 17-mer oligonucleotide with free 5'- and 3'-hydroxyl ends.

Pricing & Availability

Thermo Scientific™ dITP Solution (100 mM)

Thermo Scientific dITP (2'-deoxyinosine 5'-triphosphate) is a highly stable nucleotide supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

Promotions Available

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix

Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Content And Storage	Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form	Liquid
InhibitorToleranceSimple	High
Concentration	4X
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 5-plex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	8 hr., Assembled Reaction
Polymerase	Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format	Single-Tube
Passive Reference Dye	ROX (Pre-mixed)
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Antibody-based
Shelf Life	Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line	TaqPath™

Thermo Scientific™ RevertAid H Minus First Strand cDNA Synthesis Kit

Thermo Scientific RevertAid H Minus First Strand cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Format	Kit
Reverse Transcriptase	RevertAid H Minus
Size (Final Product)	Up to 13 kb
Reaction Format	Separate components
Final Product Type	First-Strand cDNA
Reaction Speed	60 min.
Reagent Type	Reverse Transcription
Ribonuclease H Activity	Reduced
Optimal Reaction Temperature	42°C to 45°C
Technique	Reverse Transcription
Starting Material	RNA
For Use With (Application)	Real Time PCR (qPCR),RT-PCR

Thermo Scientific™ dUTP Solution (100 mM)

Thermo Scientific dUTP (2'-deoxyuridine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Pricing & Availability

Applied Biosystems™ Oligo d(T)₁₆ (50 μM)

Invitrogen Oligo(dT)₁₆ Primer is a homo-oligomeric deoxyribonucleotide poly(dT) that is non-phosphorylated and chemically synthesized.

Pricing & Availability

Thermo Scientific™ Phusion U Hot Start PCR Master Mix

Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
Polymerase	Phusion U Hot Start DNA Polymerase
GC-Rich PCR Performance	Medium
Content And Storage	• Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl₂ • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C.
Fidelity (vs. Taq)	25X
Size (Final Product)	20 kb or less
Reaction Format	SuperMix or Master Mix
Product Type	Hot Start PCR Master Mix
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
For Use With (Application)	Hot-start PCR

100

Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™

Pricing & Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	Medium
Content And Storage	Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use.
Form	Liquid
InhibitorToleranceSimple	High
For Use With (Application)	Microbial Detection,Gene Expression,DNA Quantitation,Presence/Absence
PCR Method	1-step RT-qPCR
Multiplex Capability	Up to 6-plex
Detection Method	Primer-probe
Includes	dUTP
Thermostability	8 hr., Assembled Reaction
Polymerase	Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start
Format	Single-Tube
Passive Reference Dye	None
Reaction Speed	Fast or Standard
Target Specificity	RNA only, Both RNA and DNA
Hot Start	Antibody-based
Shelf Life	Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt
Product Line	TaqPath™

101

Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX

VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.

Pricing & Availability
Specifications

Polymerase	AmpliTaq™ Fast DNA Polymerase
Activation Time	5 min.
Final Product Type	Mastermix
Form	Liquid
Reaction Speed	Fast
Label or Dye	ROX
Hot Start	Built-in Hot Start
Concentration	2X
Starting Material	DNA/RNA
Compatibility	TaqMan™
For Use With (Application)	Animal Health
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe

102

Invitrogen™ Bio-16-UTP (10 mM)

Ambion Bio-16-UTP 10 mM (Biotinylated UTP) is intended for use as substrate in in vitro transcription reactions.

Pricing & Availability

103

Promotions Available

Thermo Scientific™ DNA Polymerase I (10 U/μL)

DNA Polymerase I is a template-dependent DNA polymerase which catalyzes 5'→3' synthesis of DNA.

Pricing & Availability
Specifications

Polymerase	DNA Polymerase I
Description	DNA Polymerase I
Concentration	10 U/μL

104

Invitrogen™ Bio-11-UTP (10 mM)

Ambion Bio-11-UTP 10 mM (Biotinylated UTP) is intended for use as substrate in in vitro transcription reactions.

Pricing & Availability

105

Applied Biosystems™ AgPath-ID™ One-Step RT-PCR Reagents with manual

AgPath-ID™ One-Step RT-PCR Reagents are designed for sensitive, robust amplification of RNA targets using a rapid single-tube TaqMan™ real-time reverse transcription PCR (RT-PCR) strategy.

Pricing & Availability
Specifications

GC-Rich PCR Performance	High
Fidelity (vs. Taq)	25 X
Description	Works on all AB platforms, but is optimized for the 7500.
Reverse Transcriptase	ArrayScript™
Concentration	25X
PCR Method	1-step RT-qPCR
Detection Method	Primer-probe
Polymerase	AmpliTaq Gold DNA Polymerase
Format	Tube
Product Type	One-Step RT-PCR Reagent
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 System
Reaction Speed	Standard
Product Line	AgPath-ID™, Ambion™

PCR Equipment and Supplies

Filtered Search Results

Narrow Results

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ DNA Polymerase I (10 U/μL) GSA/VA ContractAvailable on GSA/VA contract for Federal Government customers.Learn More

Thermo Scientific™ RevertAid H Minus Reverse Transcriptase (200 U/μL)

Thermo Scientific™ DNA Polymerase I (10 U/μL)